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Poster Presentation
Indian Institute of Technology
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Anu Kadyan Indian Institute of Technology Delhi, India

Abstract :

In the fast-growing world with the ever-increasing need for alternative solvents, ionic liquids are being explored widely in almost all areas of chemistry. Ionic liquids are room-temperature molten salts, composed of ions with notable physicochemical properties, such as, good thermal stability, high solubility, negligible vapor pressure, and non-flammability, among others. In the current energy scenario, lithium-ion batteries have proven to be a promising choice for mobile applications. But to expand its applications to large-scale, we have to cope-up with some of the limitations. Thermal stability is a major issue in the currently used electrolytes, in lithium ion batteries. While the organic solvents had their own limitations, ionic liquids, because of their desirable properties, have drawn much attention from researchers as alternative electrolytes for lithium-ion batteries. To further develop and improve this new alternative class of (ionic liquid + Li salt) electrolyte system for industrial and commercial purpose, knowledge of diffusion within such systems is of utmost importance. We present a detailed investigation of fluorescence quenching of a model solute pyrene by an electron/charge acceptor quenching agent nitromethane dissolved in [1-ethyl-3-methylimidazolium bis(trifluoromethyl sulfonyl)imide ([emim][Tf2N]) + lithium bis(trifluoromethyl sulfonyl) imide (LiTf2N)] mixtures in the temperature range (298.15 to 358.15 )K. Various equilibrium quenching constants as well as bimolecular quenching rate constants are obtained and related to the diffusion behavior within ([emim][Tf2N] + LiTf2N) system. The result is correlated with the results from fluorescence correlation spectroscopy using a different probe. Details of diffusion behavior showing versatility of (ionic liquid + Li salt) systems are established.

Poster Presentation
Kaohsiung Medical University
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Chun-Hsien Chen is currently studying Department of Fragrance and Cosmetic Science master`s program the second grade at Kaohsiung Medical University. He has completed his master's thesis of determination of finasteride and relative metabolites by capillary electrophoresis in 2017. In the two year master career, he also participated in the presentation of poster and oral at other international conferences.

Abstract :

Androgenetic alopecia leads to hair follicle miniaturization brought on Dihydrotestosterone (DHT) which is converted from testosterone by 5?-reductase. In the treatment of androgenetic alopecia, finasteride binds irreversibly to type II 5?-reductase and inhibits the conversion of testosterone to DHT. Capillary electrophoresis (CE) is applied to establish an analytical method aimed to investigate the therapeutic effect in individuals by monitoring the concentrations of finasteride and its metabolites, finasteride 2-(2-methylpropanol)amide (M1) and finasteride carboxylic acid (M3). The on-line preconcentration technique is applied to improve stacking effect on detection of analytes. Three stacking peaks are obse

Poster Presentation
National Tsing Hua University
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Hong-Lin Chan is head of the National Tsing-Hua University (Taiwan) for Quantitative Proteomics Group and has 10 years of experience in proteomic method development and application. Dr. Chan received his PhD degree from University College, University of London in 2005. After 2 year post-doctoral training in the wolfson institute for Biomedical Sciences, he took the current professorship from National Tsing-Hua University in Taiwan. Dr. Chan’s group has expertise in the preparation, separation and quantification of proteins and post-translational modifications using mass spectrometry and other methods. Dr Chan was one of the first users of 2D-DIGE technology which is routinely used for protein expression profiling and the group has also established platforms which perform quantitative phosphoproteomics and redox-proteomics analysis. Dr. Chan’s group is focused on: Serum biomarker discovery, characterising redox and UV stress responses in cell models, mechanisms of cellular signalling, proteomics based studies on breast cancer, prostate cancer and drug resistance formation. Methods include cell biology facilities, 2D-DIGE/MS and quantitative 2D-LC-MS/MS expression profiling.

Abstract :

The microbiota has increasingly gained attention because of its relationship linked to the development of human diseases. Periodontal disease is one of the disorders induced by microorganisms, which cause bad breath, swollen and bleeding gums, plaque and even tooth loss. Here, we established an anaerobic MALDI Biotyper system for in vitro diagnosis of oral microbiota to monitor the distribution of bacteria in oral cavity of human. The Bruker database used in this study contains 5,989 species of bacteria were applied to MALDI-TOF to detect oral microbiota. We collected 45 specimens of saliva and subgingival area from healthy controls and periodontitis patients. We grouped the subjects to healthy, the age under 60 with periodontal disease, and the age above 60 with periodontal disease. In addition, P-113, an antimicrobial peptide which has been reported with the ability for reducing the periodontal disease was used to evaluate the microbiota in the saliva of healthy group. We have identified 126 species by using MALDI biotyper. Based on Socransky’s classification, we found that the amount of red complex (Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola) were higher in gingival specimens of the patients of periodontal disease than healthy controls. The isolates from periodontal disease and age above 60 patients have 16% abundance of red complex; On the other hand, only 2% of red complex were found in the patients with age under 60. Moreover, only three periodontal bacteria—Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), P. gingivalis (Pg)—were present in the subgingival specimens of periodotal disease. We also found Fn and Pg in the group of age above 60 increased by 8 and 6 folds as compared to the group of age under 60. For Streptococcus gordonii and S. intermedius were higher in both saliva and subgingvial area of patients than healthy controls; Actinomyces meyeri and S. constellatus, however, are only detected in the patient group, and Actinomyces odontolyticus, S. parasanguinis, and S. salivarius have twofolds abundance in healthy group than the patient group. Our results further showed that S. mitis, S. pneumonia, Veillonella parvula are significantly decreased followed by using P-113 mouthwash. Interestingly, the number of S. salivarius, a dominant species in oral bacteria and has excellent potential for use as a probiotic targeting the oral cavity, was increased two-folds after P-113 treatment. Our results demonstrated that anaerobic MALDI-TOF Biotyper system could be a useful diagnostic tool for analyzing oral microbiota. We found that oral microbiota is periodontal disease- and age-dependent. We also provided a practical hygiene by using antimicrobial peptide P113. The results provide a way for clinical diagnosis and the basis for personal medicine of therapy in the future.

Poster Presentation
National Tsing Hua University
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Biography :

Hsiu-Chuan Chou is a professor at National Tsing Hua University in Taiwan since 2016. she received her PhD degree from King’s College, University of London in 2005. Her group focuses on studying immune cell migration, podosomes dynamics as well as proteomic approaches for the phenotypic hallmark traits and cellular signaling pathways in targeting toxic effect of environment and drug resistance of cancer treatment.

Abstract :

It is generally regarded that international energy shortages cause energy prices continuing to rise further, that the production of bio-ethanol is indispensable. In the past, glucose was used to produce biofuel. However, it is controversy over using food crops to produce fuel because most alcoholic fermentation of glucose is often obtained from sugar cane, corn and other food crops. Fortunately, the second most abundant sugar, xylose, can be utilized to generate ethanol as well. Moreover, xylose can be obtained from lignocellulosic biomass, which is in the form of forestry, agricultural, and agro-industrial wastes, such as rice rods and other plant fibers. As shown in literatures, Scheffersomyces stipitis is most suitable for fermenting xylose in microaerobic environment, whereas Scheffersomyces stipitis mutant LC11S02 collected in outdoors is most suitable for fermenting xylose in anaerobic environment. Interestingly, after mutations induced by NTG (N-methyl-N-nitro-N-nitrosoguanidine) for 30 minutes, a yeast strain LC321, similar to wild-type Scheffersomyces stipitis that is able to produce a high level of ethanol under microaerobic condition, was selected from mutated 483 strains. Furthermore, protein expression profiles were distinguished among wild-mutant (LC11S02), chemical-mutant (LC321) and standard strain (BCRC21775) by means of two dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed 108 identified proteins that are associated with metabolic pathways in xylosefermenting yeasts. Taken together, this study has the potential to provide new insights for biofuel production by efficient xylose utilization from plant biomass.

Poster Presentation
Università di Roma “Tor Vergata”
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Academic career 2014-until now: Associate Professor in Analytical Chemistry at the Department of Chemical Science and Technologies of the University of Rome "Tor Vergata"; 2002-2014: Senior Researcher in Analytical Chemistry; 1999: PhD Fellowship at the School of Chemical Sciences of Dublin City University (Ireland); 1998-2001: PhD in Chemistry Science; 1997: Master degree 110/110 cum laude in Industrial Chemistry Research. Her research activity is focalized on the study and development of disposable electrochemical tools for seafood toxins, development of immunosensors and interference-free biosensors based on screen printed electrodes (SPEs) in the field of environmental, cultural heritage, clinical and food analysis, using for their validation spectrophotometric and chromatographic methods. She has been involved in the She collaborates from 2006 until now with the Department of CEMIS-OULU of the University of Oulu. She received Peopleexchange- Marie Curie International Research Staff Exchange Scheme IRSES-PEOPLE-2008 at the School of Chemistry University of Melbourne (Australia). Her research work had been presented at several national and international scientific meetings. 7 chapters on international books, 38 on international scientific papers, 12 proceedings.

Abstract :

Wet cleaning of ancient papers is one of the most delicate and important steps in a conservation treatment. It allows the improvement of the optical qualities the removal of pollution, and the partial dissolution of organic substances resulting from cellulose degradation In this contest, a new wet cleaning technique based on the use of a rigid hydrogel of Gellan gum has been recently developed. This gel is able to gradually release the water contained within their polymer network, also to absorb the water-soluble degradation products present on paper. This compound is rigid and therefore its application and removal are fairly simple not leaving residues on the paper surface after treatment. Moreover, it could be easily combined with selective electrochemical biosensors, suitable to monitor the cleaning process. In this way, the cleaning time could be optimized, minimizing time costs and unnecessary treatments. Here we report the results obtained by applying the proposed system to the “Breviarium Romanum ad usum Fratum Minorum”, of 18th century, highlighting the advantage and the potentiality of this new tool with respect to the traditional old paper cleaning methodologies. We also used Gellan gel as a carrier of a tuned cleaning agent, that is the enzyme proteinase K to remove animal glue from the cover of the Breviary respectively. In this system, the enzyme works as selective cleaning agent, hydrolyzing not easily removable glues into smaller fragments soluble into the gel, which, in turn, plays the role of support and removal matrix for the enzymatic products.To assess the validity of this approach, several invasive and not invasive techniques, such as, fluorescence microscopy, SEM, FTIR-ATR, HPLC have been used.

Poster Presentation
São Paulo University, Brazil
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Rodrigo Rodrigues It develops materials using the TG/DTG/DSC Thermal Analysis Techniques and TG/MS in the part of obtaining and characterizing the application of thermogravimetry to obtain nanomaterials and luminescent materials, studying kinetic methods (Ozawa) in determining the time of life of compounds. And in the study of photoluminescence applications of the excitation and emission spectra of the luminescence of rare earth elements RE. Has work with colaborations of São Paulo University USP – Brazil, Turku University – Finland and Institute of Low Temperature of Wroclaw – INTIBS – Poland

Abstract :

The complex rare earth diphenyl-4-amine sulfonate RE(DAS)3?xH2O RE = [Eu3+, Tb3+] were synthesized by precursor method and characterization by photoluminescence (PL), FTIR, TG-MS, DRX and SEM. The luminescence spectra of Eu(DAS)3?7H2O and Tb(DAS)3?2H2O complexes were measured at room temperature, and the thermoanalytical study was performed in dynamic air atmosphere, using TG-MS technique. The thermal investigation shows that in dynamic air atmosphere, the oxysulfates Eu2O2SO4 and Tb2O2SO4 were obtained at approximately 973 K from the thermal decomposition of Eu(DAS)3?7H2O and Tb(DAS)3?2H2O complexes, respectively. The PL of Eu(DAS)3?7H2O show emission spectrum with groups of narrow emission bands assigned to the 5D0?7FJ transitions (where J = 0?4), dominated by the abnormal high intensity 5D0?7F4 one (685.2 and 692.8 nm), while the emission spectrum of Tb(DAS)3?2H2O complex shows narrow emission peaks with the most intense one at 545.4 nm due to 5D4?7F5 transition. In this currently work, the FTIR, DRX, MEV, thermal investigation TG/DTG/MS and photoluminescent properties was applied in the characterization and study of RE(DAS)3?H2O RE = [Eu3+, Tb3+] complexes.

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