Mass spectrometry is an analytical technique that ionizes chemical species and kinds the ions supported their mass to charge quantitative relation. In common terms, a spectrum measures the masses of sample. Mass spectrometry is employed in many alternative fields and is applied to pure samples further as complicated mixtures. A tandem mass spectrometry is one capable of multiple rounds of mass spectrographic analysis, sometimes separated by some type of molecule fragmentation. Tandem mass spectrometer allows a spread of experimental sequences. several industrial mass spectrometers are designed to expedite the execution of such routine sequences as selected reaction Monitoring and precursor particle scanning.The first perform of mass spectrometer is as a tool for chemical analyses supported detection and quantification of ions in keeping with their mass-to-charge quantitative relation. Mass spectrometry with its low sample demand and high sensitivity, has been predominantly employed in glycobiology for characterization and elucidation of glycan structures Mass spectrometry provides a complementary methodology to HPLC for the analysis of glycan’s. Intact glycans could also be detected directly as separately charged ions by matrix-assisted optical laser desorption/ionization mass spectrometry or, following permethylation or peracetylation, by quick atom bombardment mass spectrometry. Time-resolved mass spectrometry could be a strategy in analytical chemistry that uses mass spectrometry platform to gather knowledge with temporal resolution. Implementation of TRMS builds on the flexibility of mass spectrometers to method ions at intervals sub-second duty cycles. It usually needs the utilization of made-to-order experimental setups.