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Filip Cuyckens is a Scientific Director & Fellow at Janssen R&D in Beerse, Belgium. He is responsible for Analytical Sciences in the Pharmacokinetics, Dynamics & Metabolism (PDM) Department. Analytical Sciences PDM consists of Bio-transformations, focusing on metabolite profiling and identification of discovery to late development compounds, and Discovery & Exploratory Bioanalysis, focusing on quantification of drug candidates, metabolites and biomarkers in biological matrices. He earned a Pharmacist Degree in 1998, a degree in Industrial Pharmacy in 2002 and a PhD in Pharmaceutical Sciences in 2003. He has (co-) authored more than 60 publications, is a Member of the Associate Editorial Board of Rapid Communications in Mass Spectrometry and Board Member of the Belgian Society for Mass Spectrometry


High-Resolution Mass Spectrometry (HRMS) is already for many years, the analytical technique of choice for metabolite profiling and identification. While HRMS systems used to be utilized only by a few highly experienced mass spectrometrists, the performance improvements, as well as reduction in price and foot print and improving user friendliness made in the last decade, resulted in an ever growing popularity of this technique in different application areas. The new generation highresolution mass spectrometry (HRMS) systems, now also, offer the right performance for quantitative analyses, i.e., sensitivity, dynamic range, resolution, accuracy and scan-to-scan reproducibility, making them a worthwhile alternative for the ‘golden standard’ triple quadrupole MS systems. This provides a huge potential since quantitative and qualitative (quan-qual) information can be obtained from one analysis but also requires a different mindset and expertise to make the right choices and compromises to obtain the best results. The advantages and challenges of quantitative HRMS and quan-qual analyses will be discussed. While more and more smaller, cheaper and user friendly HRMS systems are available for quantitative and quan/ qual analyses, the high end HRMS instruments are further advancing in MS resolution or providing additional capabilities; thanks to combinations with other analytical techniques such as ion mobility separation and infrared spectroscopy.

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