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Nobuyuki Ichinose
Kyoto Institute of Technology, Japan

Focusing of a nanosecond laser pulse (?200 ?J) into aqueous solutions with an objective lens generates a high temperature plasma by dielectric breakdown, which induces generation of shockwave. Propagation of the shockwave with a high pressure causes linear and non-linear effects on the solute or dispersed particles. Time-resolved fluorescence spectroscopic observation under microscope has revealed that the shockwave affects local concentration of solutes due to a sub-mm movement of the molecules/particles with a near-sonic velocity in water. Combination of a ?100?m capillary to confine the shockwave propagation into one-dimension and a collagen gel to control the holding and releasing of the loaded molecules/particles made their movement give a spot as if they were brought by a laminar flow. The distance travelled of a few tens to hundreds ?m by the fluorescent-labeled proteins, DNAs, and polysaccharides or CdSe nanoparticles was found to be molecular type- and sizedependent. This technique (laser-induced shockwave chromatography) can avoid unwanted adhesion onto the solid stationary phase and will be applicable to prompt analysis to study aggregation/polymerization phenomena of biomolecules

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